Recombinant OCT4 anticorps (AA 1-134)

N° du produit ABIN7126816

Cet anticorps anti-OCT4 est un anticorps Lapin Monoclonal détectant OCT4 dans IHC (f). Adapté pour Humain.

Aperçu rapide pour Recombinant OCT4 anticorps (AA 1-134) (ABIN7126816)

Antigène: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp OCT4 est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-OCT4 anticorps

Épitope: AA 1-134

Specificité: OCT4 is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells.OCT4 is a key regulator of self-renewal in embryonic stem cells, its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies.OCT4 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testes. OCT4 Ab is useful in the identification of primary as well as metastatic germ cell tumors.

Réactivité croisée (Details): Human

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment of human OCT-4/POU5F1 protein (around aa1-134) (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Raji or U-937cells. Human seminoma or testicular carcinoma

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur OCT4

Antigène: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Autre désignation: POU5F1

Sujet: OCT3, OCT3A, OCT4, OCT4A, OCT-3/4, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, octamer-binding transcription factor-3, OTF-3, OTF3, OTF4, PO5F1, POU class 5 homeobox 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, POU5F1,OCT-4 (POU5F1) (Germ CellTumor Marker)
Cellular localisation: Nucleus

Poids moléculaire: 38.6kDa

ID gène: 5460, 249184

UniProt: Q01860

Pathways: Stem Cell Maintenance